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dual index primer pairs  (New England Biolabs)


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    New England Biolabs dual index primer pairs
    Dual Index Primer Pairs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/index+primers/NEBNext+Multi+Olig+IL+96+Dual+index+prim/bio_rxiv__64898__2026__05__08__723508-214-28-32
    Average 97 stars, based on 2751 article reviews
    dual index primer pairs - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Amplification:

    Article Title: Argonaute-HNH filaments triggered by invader DNA confer bacterial immunity.
    Article Snippet: The resulting adapter (2.5 l of 10 M solution) was ligated to the purified 3’-dA overhanged cleavage products in 50 l samples containing 5 l of 1 U/l T4 DNA ligase and 5 l of 10x T4 DNA ligase buffer (NEB) for 16 h at 22 °C. .. The resulting samples were amplified with dual index primers (NEBNext Multiplex Oligos for Illumina) and purified with Agencourt AMPure XP beads. .. Next, sequencing primers were added using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina.

    Article Title: Argonaute-HNH filaments triggered by invader DNA confer bacterial immunity
    Article Snippet: Next, sequencing primers were added using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina. .. The resulting samples were amplified with dual index primers (NEBNext Multiplex Oligos for Illumina) and purified with Agencourt AMPure XP beads. .. PCR products were sequenced on a NovaSeq 6000 System (Illumina) in the 100 bp paired-end mode.

    Article Title: ADNP regulates chromatin architecture and lineage fidelity during neural differentiation.
    Article Snippet: Size selection for fragments >150 bp was performed using AMPure XP magnetic beads (Beckman Coulter). .. Libraries were PCR amplified with dual index primers (NEB #E7600) using Q5 DNA polymerase (NEB #M0491) and purified with MinElute. .. Libraries for Hi-C samples were prepared using the NEBNext Ultra II DNA Library Prep Kit with modifications according to the Arima Genomics Hi-C Kit.

    Article Title: ADNP regulates chromatin architecture and lineage fidelity during neural differentiation
    Article Snippet: Size selection for fragments >150 bp was performed using AMPure XP magnetic beads (Beckman Coulter). .. Libraries were PCR amplified with dual index primers (NEB #E7600) using Q5 DNA polymerase (NEB #M0491) and purified with MinElute. .. Libraries for Hi-C samples were prepared using the NEBNext Ultra II DNA Library Prep Kit with modifications according to the Arima Genomics Hi-C Kit.

    Multiplex Assay:

    Article Title: Argonaute-HNH filaments triggered by invader DNA confer bacterial immunity.
    Article Snippet: The resulting adapter (2.5 l of 10 M solution) was ligated to the purified 3’-dA overhanged cleavage products in 50 l samples containing 5 l of 1 U/l T4 DNA ligase and 5 l of 10x T4 DNA ligase buffer (NEB) for 16 h at 22 °C. .. The resulting samples were amplified with dual index primers (NEBNext Multiplex Oligos for Illumina) and purified with Agencourt AMPure XP beads. .. Next, sequencing primers were added using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina.

    Article Title: Niche specialization and cross-feeding interactions shaping gut microbial fiber degradation in a model omnivore
    Article Snippet: .. Host and microbial mRNA libraries were constructed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB), following instructions to obtain 300 bp inserts flanked by Illumina-compatible index primers (NEBNext® Multiplex Oligos for Illumina®). .. Following size and quality verification with the Bioanalyzer, the barcoded samples were pooled together and sent to Novogene (Sacramento, CA, USA) for Illumina NovaSeq sequencing.

    Article Title: Argonaute-HNH filaments triggered by invader DNA confer bacterial immunity
    Article Snippet: Next, sequencing primers were added using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina. .. The resulting samples were amplified with dual index primers (NEBNext Multiplex Oligos for Illumina) and purified with Agencourt AMPure XP beads. .. PCR products were sequenced on a NovaSeq 6000 System (Illumina) in the 100 bp paired-end mode.

    Article Title: Transcription factor ZNF263 primes human embryonic stem cells for pluripotency dissolution and lineage commitment
    Article Snippet: Following the cDNA generation, fragmentation, adaptor ligation, and amplification with NEBNext Ultra II RNA Library Prep Kit (New England BioLabs, Cat# E7775S), the libraries were purified with AMPure XP beads (Beckman Coulter, Cat#A63880). .. Index primers from NEBNext Multiplex Oligos for Illumina (Dual Index Primers set 1) (New England BioLabs, Cat#E7600S) were used to enable index incorporation during library amplification. ..

    Article Title: Improved chromosome-level genome assembly of the American cockroach, Periplaneta americana
    Article Snippet: Total RNA was incubated with 1 μL TURBO DNase enzyme (Invitrogen) and 5 μL TURBO DNase reaction buffer for 20 min at 37 °C to destroy contaminating DNA, then purified using the column-based EZNA MicroElute RNA Clean-Up Kit (Omega-Biotek) into 30 μL nuclease-free water. .. RNA quality was assessed with an Agilent Bioanalyzer and samples were stored at −80 °C until library preparation, which was performed using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB), the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB), and Illumina-compatible index primers (NEBNext® Multiplex Oligos for Illumina). .. In all, 24 separate libraries (3 per insect) with 300 bp inserts were sent for 150 bp paired-end Illumina NovaSeq sequencing at Novogene (Sacramento, CA, USA).

    Article Title: Transcription factor ZNF263 primes human embryonic stem cells for pluripotency dissolution and lineage commitment.
    Article Snippet: Following the cDNA generation, fragmentation, adaptor ligation, and amplificationwithNEBNextUltra II RNALibraryPrepKit (New EnglandBioLabs, Cat# E7775S), the librarieswere purifiedwithAMPure Nature Communications | (2025) 16:9757 14 XP beads (Beckman Coulter, Cat#A63880). .. Index primers from NEBNext Multiplex Oligos for Illumina (Dual Index Primers set 1) (New England BioLabs, Cat#E7600S) were used to enable index incorporation during library amplification. ..

    Purification:

    Article Title: Argonaute-HNH filaments triggered by invader DNA confer bacterial immunity.
    Article Snippet: The resulting adapter (2.5 l of 10 M solution) was ligated to the purified 3’-dA overhanged cleavage products in 50 l samples containing 5 l of 1 U/l T4 DNA ligase and 5 l of 10x T4 DNA ligase buffer (NEB) for 16 h at 22 °C. .. The resulting samples were amplified with dual index primers (NEBNext Multiplex Oligos for Illumina) and purified with Agencourt AMPure XP beads. .. Next, sequencing primers were added using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina.

    Article Title: Argonaute-HNH filaments triggered by invader DNA confer bacterial immunity
    Article Snippet: Next, sequencing primers were added using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina. .. The resulting samples were amplified with dual index primers (NEBNext Multiplex Oligos for Illumina) and purified with Agencourt AMPure XP beads. .. PCR products were sequenced on a NovaSeq 6000 System (Illumina) in the 100 bp paired-end mode.

    Article Title: ADNP regulates chromatin architecture and lineage fidelity during neural differentiation.
    Article Snippet: Size selection for fragments >150 bp was performed using AMPure XP magnetic beads (Beckman Coulter). .. Libraries were PCR amplified with dual index primers (NEB #E7600) using Q5 DNA polymerase (NEB #M0491) and purified with MinElute. .. Libraries for Hi-C samples were prepared using the NEBNext Ultra II DNA Library Prep Kit with modifications according to the Arima Genomics Hi-C Kit.

    Article Title: ADNP regulates chromatin architecture and lineage fidelity during neural differentiation
    Article Snippet: Size selection for fragments >150 bp was performed using AMPure XP magnetic beads (Beckman Coulter). .. Libraries were PCR amplified with dual index primers (NEB #E7600) using Q5 DNA polymerase (NEB #M0491) and purified with MinElute. .. Libraries for Hi-C samples were prepared using the NEBNext Ultra II DNA Library Prep Kit with modifications according to the Arima Genomics Hi-C Kit.

    Construct:

    Article Title: Niche specialization and cross-feeding interactions shaping gut microbial fiber degradation in a model omnivore
    Article Snippet: .. Host and microbial mRNA libraries were constructed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB), following instructions to obtain 300 bp inserts flanked by Illumina-compatible index primers (NEBNext® Multiplex Oligos for Illumina®). .. Following size and quality verification with the Bioanalyzer, the barcoded samples were pooled together and sent to Novogene (Sacramento, CA, USA) for Illumina NovaSeq sequencing.

    Polymerase Chain Reaction:

    Article Title: ADNP regulates chromatin architecture and lineage fidelity during neural differentiation.
    Article Snippet: Size selection for fragments >150 bp was performed using AMPure XP magnetic beads (Beckman Coulter). .. Libraries were PCR amplified with dual index primers (NEB #E7600) using Q5 DNA polymerase (NEB #M0491) and purified with MinElute. .. Libraries for Hi-C samples were prepared using the NEBNext Ultra II DNA Library Prep Kit with modifications according to the Arima Genomics Hi-C Kit.

    Article Title: ADNP regulates chromatin architecture and lineage fidelity during neural differentiation
    Article Snippet: Size selection for fragments >150 bp was performed using AMPure XP magnetic beads (Beckman Coulter). .. Libraries were PCR amplified with dual index primers (NEB #E7600) using Q5 DNA polymerase (NEB #M0491) and purified with MinElute. .. Libraries for Hi-C samples were prepared using the NEBNext Ultra II DNA Library Prep Kit with modifications according to the Arima Genomics Hi-C Kit.

    Library Amplification:

    Article Title: Transcription factor ZNF263 primes human embryonic stem cells for pluripotency dissolution and lineage commitment
    Article Snippet: Following the cDNA generation, fragmentation, adaptor ligation, and amplification with NEBNext Ultra II RNA Library Prep Kit (New England BioLabs, Cat# E7775S), the libraries were purified with AMPure XP beads (Beckman Coulter, Cat#A63880). .. Index primers from NEBNext Multiplex Oligos for Illumina (Dual Index Primers set 1) (New England BioLabs, Cat#E7600S) were used to enable index incorporation during library amplification. ..

    Article Title: Transcription factor ZNF263 primes human embryonic stem cells for pluripotency dissolution and lineage commitment.
    Article Snippet: Following the cDNA generation, fragmentation, adaptor ligation, and amplificationwithNEBNextUltra II RNALibraryPrepKit (New EnglandBioLabs, Cat# E7775S), the librarieswere purifiedwithAMPure Nature Communications | (2025) 16:9757 14 XP beads (Beckman Coulter, Cat#A63880). .. Index primers from NEBNext Multiplex Oligos for Illumina (Dual Index Primers set 1) (New England BioLabs, Cat#E7600S) were used to enable index incorporation during library amplification. ..

    Isolation:

    Article Title: Improved chromosome-level genome assembly of the American cockroach, Periplaneta americana
    Article Snippet: Total RNA was incubated with 1 μL TURBO DNase enzyme (Invitrogen) and 5 μL TURBO DNase reaction buffer for 20 min at 37 °C to destroy contaminating DNA, then purified using the column-based EZNA MicroElute RNA Clean-Up Kit (Omega-Biotek) into 30 μL nuclease-free water. .. RNA quality was assessed with an Agilent Bioanalyzer and samples were stored at −80 °C until library preparation, which was performed using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB), the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB), and Illumina-compatible index primers (NEBNext® Multiplex Oligos for Illumina). .. In all, 24 separate libraries (3 per insect) with 300 bp inserts were sent for 150 bp paired-end Illumina NovaSeq sequencing at Novogene (Sacramento, CA, USA).



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